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1.MSC exosomes had minimal effect on the polarization of CD3/CD28-costimulated CD4+T cells to CD4+CD25+FoxP3+ tregs
2.MSC
exosomes increased polarization of CD4+T cells to CD4+CD25+FoxP3+ tregs in the presence of allogeneic CD11c+ cells
3.MSC exosomes alleviated GVHD and improved survival of mice with an increase in human CD4+CD25+CD127low/– tregs
Figure 1. Anti-CD3/CD28 mAb activation of CD4+T cells andTreg induction by MSC exosome. CD4+T cells were purified from C57BL/6 mouse spleens and incubated with or without (w/wo) MSC exosomes (10 or 30 ug/mL) or TGF-β1 (10 ηg/mL) for CD4+CD25+Foxp3+ Treg cells in the presence of anti-CD3 mAb (5 3g/mL) and anti-CD28 mAb (5 g/mL) for 6 days. The cells were then harvested and analyzed by FACS for the presence of (A) CD4+CD25+ cells or (B) CD4+CD25+Foxp3+Treg cells. The percentage of CD4+CD25+ cells or CD4+CD25+Foxp3+ Treg cells was normalized to that of CD4+T cells exposed to only anti-CD3 mAb (5 g/mL) and anti-CD28 mAb (5 g/mL) (untreated control). Each bar represents the mean (±SD) of three independent assays performed in duplicate or triplicate. **P<0.001.
